Functional analysis of Drosophila factor 5 (TFIIF), a general transcription factor.
نویسندگان
چکیده
منابع مشابه
Analysis of cDNA encoding Drosophila transcription initiation factor TFIIF alpha (RAP74).
At least five general initiation factors (1'HIB, TFIID, TFIIE, Thllh, and TMiH) are essential to accurately initiate transcription in the RNA polymerase II system. TFIIF, the subject of this report, binds to RNA polymerase II and is essential for its stable assembly into a preformed promoter-ThllD-ThllB complex. Moreover, TFIIF plays an important role in transcription elongation as well as tran...
متن کاملPosition of the general transcription factor TFIIF within the RNA polymerase II transcription preinitiation complex.
The RNA polymerase (pol) II general transcription factor TFIIF functions at several steps in transcription initiation including preinitiation complex (PIC) formation and start site selection. We find that two structured TFIIF domains bind Pol II at separate locations far from the active site with the TFIIF dimerization domain on the Pol II lobe and the winged helix domain of the TFIIF small sub...
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Extended Abstract Introduction and Objective: Medicinal plants have many applications in the treatment of humans, among which chamomile is one of the most valuable and widely used medicinal plants in the world. One of the most important breeding programs is the recognition of genetic diversity for the initial evaluation of plant masses. For this purpose, a study of genetic diversity and classi...
متن کاملRNA polymerase III-specific general transcription factor IIIC contains a heterodimer resembling TFIIF Rap30/Rap74
Transcription of tRNA-encoding genes by RNA polymerase (Pol) III requires the six-subunit general transcription factor IIIC that uses subcomplexes τA and τB to recognize two gene-internal promoter elements named A- and B-box. The Schizosaccharomyces pombe τA subcomplex comprises subunits Sfc1, Sfc4 and Sfc7. The crystal structure of the Sfc1/Sfc7 heterodimer reveals similar domains and overall ...
متن کاملTfg3, a subunit of the general transcription factor TFIIF in Schizosaccharomyces pombe, functions under stress conditions.
TFIIF is a general transcription factor (GTF) that binds to RNA polymerase II (pol II) for subsequent recruitment of pol II to a promoter. TFIIF of Saccharomyces cerevisiae contains a small subunit, designated Tfg3, in addition to two conserved subunits, TFIIFalpha (Tfg1) and TFIIFbeta (Tfg2). In this study, we characterized Tfg3 of Schizosaccharomyces pombe. Using Tfg3 fused to green fluoresce...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)36864-3